Analysis of four tylosin biosynthetic genes from the tylLM region of the Streptomyces fradiae genome |
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| Institution: | 1. KU Leuven, University of Leuven, Department of Pharmaceutical and Pharmacological Sciences, Pharmaceutical Analysis, Herestraat 49, O&N2, PB 923, B-3000 Leuven, Belgium;2. Mekelle University, College of Health Sciences, Department of Pharmacy, P.O. Box 1871, Mekelle, Ethiopia;3. Topfond Pharmaceutical Co, Ltd., No.2 Guangming Road, Zhumadian, Henan, China;1. University of Chinese Academy of Sciences, Beijing, 100049, China;2. Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, 300308, China;3. Institute of Biological Chemistry, Academia Sinica, Taipei, 11529, Taiwan;4. State Key Laboratory of Biocatalysis and Enzyme Engineering, Hubei Collaborative Innovation Center for Green Transformation of Bio-Resources, Hubei Key Laboratory of Industrial Biotechnology, School of Life Sciences, Hubei University, Wuhan, 430062, China;1. Department of Ecology, Institute of Hydrobiology, School of Life Science and Technology, Jinan University, Guangzhou 510632, PR China;2. Department of Molecular Structural Biology, Institute for Biochemistry, University of Greifswald, Felix-Hausdorff-Strasse 4, D-17489 Greifswald, Germany;3. Laboratory of “Energetic Metabolism of Streptomyces”, Institute of Genetics and Microbiology, University of Paris-Sud 11, France;1. College of Life Sciences, Huzhou Teachers College, Huzhou 313000, People''s Republic of China;2. College of Life Sciences, Jiujiang University, Jiujiang 332005, People''s Republic of China;1. UMR 7365, Ingénierie Moléculaire et Physiopathologie Articulaire (IMoPA), CNRS-Université de Lorraine, Biopôle de l’Université de Lorraine, Campus Biologie Santé, 9 Avenue de la Forêt de Haye, CS 50184, 54505 Vand?uvre-lès-Nancy Cedex, France |
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| Abstract: | The tylLM region of the tylosin biosynthetic gene cluster of Streptomyces fradiae contains four open reading frames (orfs1*–4*). The function of the orf1* product is not known. The product of orf2* (tylM2) is the glycosyltransferase that adds mycaminose to the 5-hydroxyl group of tylactone, the polyketide aglycone of tylosin (Ty). A methyltransferase, responsible for 3-N-methylation during mycaminose production, is encoded by orf3* (tylM1). The product of orf4* (ccr) is crotonyl-CoA reductase, which converts acetoacetyl-CoA to butyryl-CoA for use as a 4C extender unit during tylactone production. |
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