Molecular cloning of a putative serotonin receptor gene from barnacle,Balanus amphitrite |
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| Institution: | 1. Jiangsu Key Laboratory of Marine Biotechnology/College of Marine Science/Co-Innovation Center of Jiangsu Marine Bio-industry Technology, Huaihai Institute of Technology, Lianyungang 222005, China;2. Simon F. S. Li Marine Science Laboratory, School of Life Sciences, The Chinese University of Hong Kong, Shatin, N.T., Hong Kong, China;3. Institute of Marine Biology, National Taiwan Ocean University, Keelung 202, Taiwan;4. The Mina and Everard Goodman Faculty of Life Sciences, Bar-Ilan University, Ramat-Gan 52900, Israel;5. Biodiversity Research Center, Academia Sinica, Taipei 115, Taiwan;1. University of Warmia & Mazury, Faculty of Environmental Sciences, M. Oczapowskiego 5, 10–719 Olsztyn, Poland;;2. The State School of Higher Professional Education in Elbląg, Institute of Technology, Wojska Polskiego 1, 82–300 Elbląg, Poland |
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| Abstract: | We isolated a putative serotonin receptor gene from a genomic library of the barnacle, Balanus amphitrite Darwin, using an NcoI fragment of the barnacle G protein-coupled receptor gene that is homologous to the α2-adrenoceptor. The cloned genomic DNA had no intron and specified an open reading frame of 1137 base pairs encoding 379 amino acids (aa). The predicted aa sequence has a typical seven hydrophobic transmembrane spanning region and a consensus G protein-binding motif. This receptor was most homologous to the human 5HT1A receptor and closely related to other 5HT1 receptor subtypes. |
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