Cloning,sequencing and expression of the two genes encoding the mitochondrial single-stranded DNA-binding protein in Xenopus laevis |
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| Institution: | 1. Laboratoire Evolution et Diversité Biologique (EDB), UMR5174, Bâtiment 4R1, 118 Route de Narbonne, 31062 Toulouse cedex 9, France;2. Laboratoire Ecologie, Evolution, Interactions des Systèmes Amazoniens (LEEISA), USR3456 Cayenne, French Guiana;3. Association Kwata, 16 avenue Pasteur, Cayenne, French Guiana;4. Institut Pasteur de la Guyane, 23 avenue Pasteur, Cayenne, French Guiana;5. Instituto de Pesquisas Cientificas e Tecnológicas do Amapá (IEPA) – Nucleo de Biodiversidade (NUBIO), Laboratório de Herpetologia, Rodovia Juscelino Kubitschek, s/n, Distrito da Fazendinha, Macapá, AP, Brazil;6. Universidade Federal de Pernambuco, Centro de Biocências, Departamento de Zoologia, Av. Professor Moraes Rego, s/n, 50670-901 Recife, PE, Brazil;7. Universidade de São Paulo, Instituto de Biociências, Departamento de Zoologia, Caixa Postal 11.461, CEP 05508-090 São Paulo, SP, Brazil;8. University of Mississippi, Department of Biology, Oxford, 507 Shoemaker Hall, University, MS 38677, USA;1. New York University School of Medicine, Nelson Institute of Environmental Medicine, Tuxedo, NY, USA;2. San Joaquin Valley Air Pollution Control District, Fresno, CA, USA;3. Clinical Research Center, Department of Medicine, Fresno, CA, USA;4. Lamont-Doherty Earth Observatory, Columbia University, Palisades, NY, USA;5. Center for Health and the Environment, University of California-Davis, Davis, CA, USA;1. Department of Orthopaedic Surgery, William Beaumont Army Medical Center, El Paso, TX;2. Department of Orthopaedic Surgery, Brigham and Women''s Hospital, Boston, MA;1. Irstea, UR LISC Laboratoire d''ingénierie des systèmes complexes, 9 avenue Blaise Pascal - CS 20085, Aubière, 63178, France;2. Université Clermont Auvergne, Laboratoire Microorganismes: Génome et Environnement, BP, 10448, Clermont-Ferrand, France;3. CNRS, UMR 6023, Laboratoire Microorganismes: Génome et Environnement, BP, 80026, Aubière, France |
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| Abstract: | In Xenopus laevis the single-stranded DNA binding protein imported into the mitochondria consists of two highly related polypeptides. The establishment of the genomic nucleotide sequences reveals that they are encoded by two different genes, XLSSB1 and XLSSB2. The deduced amino acid sequence is identical to the direct amino acid sequence determined by Edman degradation of the mitochondrial polypeptides Ghrir, R., Lecaer, J.P., Dufresne, C. and Gueride, M. (1991) Primary structure of the two variants of Xenopus laevis mtSSB, a mitochondrial DNA binding protein. Arch. Biochem. Biophys. 291, 395–400]. Both genes are organized in seven exons and six introns, the sequence of the peptide leader is interrupted by an intervening sequence (intron 2). The exon/intron junctions are in exactly conserved positions, splitting the same codon. A high level of identity is observed between corresponding introns of the two genes over part or most of their lengths. Structural features of intronic sequences reveal multiple rearrangements and exchanges during the evolution of X. laevis species. A CCAAT box and the potential regulatory elements NRF-2 and Sp 1 are observed in the 5′-flanking region of both genes. During oogenesis, XLSSB gene expression is correlated with the replicative activity of the mitochondrial DNA. |
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