Cloning and chromosomal mapping of four putative novel human G-protein-coupled receptor genes |
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| Affiliation: | 1. Division of Functional Pathology, Department of Pathology, Hyogo College of Medicine, Nishinomiya, Hyogo 663-8501, Japan;2. Department of Dentistry and Oral Surgery, Hyogo College of Medicine, Nishinomiya, Hyogo 663-8501, Japan;1. University of Oklahoma Health Sciences Center, Oklahoma City;2. Georgia Cancer Specialists, Atlanta;3. SCRI, Tennessee Oncology, PLLC, Nashville;4. Arqule, Inc., Woburn;5. BioMarin Pharmaceutical, Inc., Novato;6. St Luke''s Medical Center, Houston, USA;1. Institute of Biochemistry, Food Science and Nutrition, The Hebrew University of Jerusalem, Rehovot, Israel;2. Monell Chemical Senses Center, Philadelphia, PA, USA;3. College of Dentistry, New York University, New York, NY, USA;4. Department of Biological Regulation, The Weizmann Institute of Science, Rehovot, Israel;1. The Department of Cardiology, Patras University Hospital, Rion, Patras, Greece;2. Cardiovascular Division, Department of Medicine, Brigham and Women''s Hospital Heart & Vascular Center and Harvard Medical School, Boston, MA;3. Kerckhoff Heart and Thoraxcenter, Bad Nauheim Medical Clinic I, University of Giessen, Giessen, Germany;4. Université Paris-Diderot, Sorbonne Paris-Cité, Paris, France;5. Département Hospitalo-Universitaire FIRE, Hôpital Bichat, AP-HP, Paris, France;6. INSERM U1148, NHLI, Paris, France;7. Imperial College, ICMS, Royal Brompton Hospital, London, UK;8. Columbia University Medical Center and the Cardiovascular Research Foundation, New York, NY |
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| Abstract: | We report the discovery of four novel human putative G-protein-coupled receptor (GPCR) genes. Gene GPR20 was isolated by amplifying genomic DNA with oligos based on the opioid and somatostatin related receptor genes and subsequent screening of a genomic library. Also, using our customized search procedure of a database of expressed sequence tags (dbEST), cDNA sequences that partially encoded novel GPCRs were identified. These cDNA fragments were obtained and used to screen a genomic library to isolate the full-length coding region of the genes. This resulted in the isolation of genes GPR21, GPR22 and GPR23. The four encoded receptors share significant identity to each other and to other members of the receptor family. Northern blot analysis revealed expression of GPR20 and GPR22 in several human brain regions while GPR20 expression was detected also in liver. Fluorescence in situ hybridization (FISH) was used to map GPR20 to chromosome 8q, region 24.3–24.2, GPR21 to chromosome 9, region q33, GPR22 to chromosome 7, region q22–q31.1, and GPR23 to chromosome X, region q13–q21.1.© 1997 Elsevier Science B.V. All rights reserved. |
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