Characterization of the H1.5 gene completes the set of human H1 subtype genes |
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| Institution: | 1. Inserm, U1144, Paris F-75006, France;2. Université Sorbonne Paris Cité, UMR-S 1144, Paris F-75006, France;3. AP-HP, GH Saint-Louis – Lariboisière – F. Widal, Pôle de Psychiatrie et de Médecine Addictologique, 75475 Paris cedex 10, France;4. Fondation FondaMental, Créteil, 94000, France;5. Département de Biostatistique et Informatique Médicale, Hôpital Saint-Louis, APHP, Paris, France;1. School of Integrative and Global Majors, University of Tsukuba, Tsukuba, Japan;2. Graduate Institute of Oral Biology, School of Dentistry, National Taiwan University, Taipei, Taiwan;3. Center for Biotechnology, National Taiwan University, Taipei, Taiwan;4. Department of Experimental Pathology, Faculty of Medicine, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8575, Japan;5. Transborder Medical Research Center, Faculty of Medicine, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8575, Japan;1. Jiangsu Key Laboratory of Drug Screening, China Pharmaceutical University, Nanjing, Jiangsu, China;2. Department of Intervention Treatment, Shenzhen People''s Hospital, Shenzhen, Guangdong, China;3. Jiangsu Center for Pharmacodynamics Research and Evaluation, China Pharmaceutical University, Nanjing, Jiangsu, China;4. Interventional Radiology Center, Zhuhai Precision Medicine Center, Zhuhai People''s Hospital of Tongji University, Zhuhai, Guangdong, China;1. Institute for Cancer Research and Molecular Medicine, Norwegian University of Science and Technology, Laboratory Center, Erling Skjalgssons Gate 1, 7491 Trondheim, Norway;2. The NNF Center for Protein Research, Faculty of Health and Medical Sciences, University of Copenhagen, Blegdamsvej 3B, 2200 Copenhagen, Denmark;3. Howard Hughes Medical Institute, Program in Cellular and Molecular Medicine, Boston Children’s Hospital, Department of Genetics, Harvard Medical School, Boston, MA 02115, United States;4. St. Olavs Hospital, Trondheim University Hospital, Clinic of Medicine, Postboks 3250 Sluppen, 7006 Trondheim, Norway;1. Department of Otorhinolaryngology, University Hospital of Larissa, Greece;2. Sleep Disorders Laboratory, University Hospital of Larissa, Greece |
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| Abstract: | The H1 histone family in mammals contains at least seven subtypes. In the past we have isolated six of the seven genes encoding these isoforms. To complete the set of the human H1 histone genes, we have designed two PCR primers deduced from a partially published sequence of the remaining histone H1 gene Carozzi et al. (1984)Science 224, 1115–1118] and from a consensus sequence which we have derived from the conserved region of human histone H1 genes. Using these primers we have amplified a 417-bp DNA fragment from total human DNA. This fragment was used for screening a human phage genomic library. Two overlapping clones were isolated. The region contains a set of 5 genes representing each of the five histone classes. In continuation of our numbering of human H1 genes, we have named this H1 gene H1.5. This gene encodes a protein almost identical to the previously published protein sequence designated H1a Ohe et al. (1986)J. Biochem. 100, 359–368]; since the changes are in a region of some uncertainty of the peptide sequencing, we conclude that the newly isolated gene codes for the H1a protein. The structures of the flanking regions of the genes except the H2B gene are typical for histone genes. They include: (1) a CCAAT element in the promotor region, (2) a TATA box and (3) a palindromic termination element. The H2B sequence shows no typical regulatory elements and no complete ORF, therefore we consider it as a pseudogene. The expression of the H1.5 gene was examined in several cell lines. |
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