Genetic map of the virulence plasmid of Salmonella enteritidis and nucleotide sequence of its replicons |
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| Affiliation: | 1. Centre of Laboratory Medicine, Zhejiang Provincial People’s Hospital, People’s Hospital of Hangzhou Medical College, 158 Shangtang Road, Hangzhou, Zhejiang 310014, China;2. Blood Center of Zhejiang Province, 789 Jianye Road, Hangzhou, Zhejiang 310052, China;1. Laboratorio Nacional Para la Investigación en Inocuidad Alimentaria (LANIIA), Centro de Investigación en Alimentación y Desarrollo, A.C. (CIAD), Carretera a El dorado km 5.5, Campo El Diez, 80110 Culiacán, Sinaloa, Mexico;2. Laboratorio de Microbiología Molecular y Bioactivos, Tecnológico Nacional de México-IT Culiacán, Juan de Dios Bátiz 310 pte, Colonia Guadalupe, 80220 Culiacán, Sinaloa, Mexico;3. Investigadoras e Investigadores por México-Centro de Investigación en Alimentación y Desarrollo, A.C. (CIAD), Laboratorio Nacional Para la Investigación en Inocuidad Alimentaria (LANIIA), Carretera a El dorado km 5.5, Campo El Diez, 80110 Culiacán, Sinaloa, Mexico;1. Instituto de Patobiología, CICVyA, Instituto Nacional de Tecnología Agropecuaria, Hurlingham 1686, Argentina;2. Laboratorio de Fisiopatogenia, Departamento de Fisiología, Instituto de Fisiología y Biofísica Bernardo Houssay (IFIBIO Houssay-CONICET), Facultad de Medicina, Universidad de Buenos Aires, Buenos Aires 1121, Argentina;3. Facultad de Agronomía, Universidad de Buenos Aires-CONICET, Buenos Aires 1427, Argentina;4. Cátedra de Microbiología, Facultad de Ciencias Veterinarias, Universidad de Buenos Aires, Buenos Aires 1427, Argentina;5. Servicio Fisiopatogenia, INEI-ANLIS “Dr. Carlos G. Malbrán”, Buenos Aires 1282, Argentina;6. Instituto de Biotecnología, CICVyA, Instituto Nacional de Tecnología Agropecuaria, Hurlingham 1686, Argentina |
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| Abstract: | Partial sequencing of a genomic library of the virulence plasmid of Salmonella enteritidis has been used to localize in the restriction map of this plasmid the genetic loci already described in other Salmonella plasmids. The comparison of the vestigial tra region with the corresponding genes in the F plasmid allowed us to define the extent of the deletions that the S. enteritidis plasmid should have suffered. The putative replicons of the plasmid, repB and repC, were isolated and both proved to be functional in Escherichia coli, but repC was segregationally unstable. The nucleotide sequence of repB showed the typical organization of RepFIIA replicons, although the similarity was lower than usual in this group of replicons. The highest homology was found with the replicon of the virulence plasmid pYVe439-80 from Yersinia enterocolitica (72.5%). Replicon repC also showed a maximum identity of 72.6% with known replicons, namely the RepFIB of pColV-K30 and P307, both virulence plasmids isolated from E. coli. We conclude that the S. enteritidis plasmid could arise from the S. typhimurium plasmid through deletions, and that they are evolutionary distant from other IncFI and IncFII plasmids. |
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