Structure of the rice glutaredoxin (thioltransferase) gene |
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| Institution: | 1. Department of Biochemistry, College of Agriculture, Kyoto Prefectural University, Shimogamo, Kyoto 606, Japan;2. Development and Research Laboratories, JCR Pharmaceuticals Co., Ltd., 2-2-10 Murotani, Nishi-ku, Kobe 651-22, Japan;1. Graduate School of Science, Kyoto University, Kyoto 606-8502, Japan;1. Department of Virology, Biological Faculty, Moscow State University, Moscow 119234, Russia;2. Belozersky Institute of Physico-Chemical Biology, Moscow State University, Moscow 119992, Russia;3. Sechenov First Moscow State Medical University, Institute of Molecular Medicine, Moscow 119991, Russia;1. Biotechnology Research Institute, Chinese Academy of Agricultural Science, Beijing 100081, China;2. Division of Applied Life Science (BK21 plus program), Plant Molecular Biology & Biotechnology Research Center, Gyeongsang National University, Jinju 660-701, Korea |
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| Abstract: | We have isolated the gene encoding a glutaredoxin in rice (Oryza sativa L.) and determined the nucleotide (nt) sequence of about a 4.2 kb long. The cloned gene (gRASC8) was found to contain four exons interrupted by three introns. The first exon begins the ATG translation start codon and the four exons code for a protein composed of 112 amino acids. The tetrapeptide -Cys-Pro-Phe-Cys- -Cys-Pro-Phe(Tyr)-Cys-] which constitutes an active site of Escherichia coli and mammalian glutaredoxins, was conserved. The nt sequence contained consensus TATA and CAAT boxes, and two polyadenylation signals. Southern blot analysis of rice genomic DNA suggests that there are two copies of the glutaredoxin genes in rice. |
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