Porin from the halophilic species Ectothiorhodospira vacuolata: cloning,structure of the gene and comparison with other porins |
| |
| Institution: | 1. Department of Crop Production and Plant Breeding, College of Agriculture, Shiraz University, Shiraz 7144165186, Iran;2. Adelaide Medical School, Faculty of Health and Medical Sciences, The University of Adelaide, Adelaide 5005, Australia;3. Institute of Biotechnology, Shiraz University, Shiraz 7144165186, Iran;4. Division of Information Technology, Engineering and the Environment, School of Information Technology and Mathematical Sciences, University of South Australia, Adelaide 5005, Australia;5. School of Biological Sciences, Faculty of Science and Engineering, Flinders University, Adelaide 5005, Australia;6. Department of Biology, University of Qom, Qom, 371514661, Iran;7. Australian Centre for Antimicrobial Resistance Ecology, School of Animal and Veterinary Sciences, The University of Adelaide, Adelaide 5005, Australia |
| |
| Abstract: | The gene coding for the anion-specific porin of the halophilic eubacterium Ectothiorhodospira (Ect.) vacuolata was cloned and sequenced, the first such gene so analyzed from a purple sulfur bacterium. It encodes a precursor protein consisting of 374 amino acid (aa)-residues including a signal peptide of 22-aa residues. Comparison with aa sequences of porins from several other members of the Proteobacteria revealed little homology. Only two regions showed local homology with the previously sequenced porins of Neisseria species, Comamonas acidovorans, Bordetella pertussis, Alcaligenes eutrophus, and Burkholderia cepacia. Genomic Southern blot hybridization studies were carried out with a probe derived from the 5′ end of the gene coding for the porin of Ect. vacuolata. Two related species, Ect. haloalkaliphila and Ect. shaposhnikovii, exhibited a clear signal, while the extremely halophilic bacterium Halorhodospira (Hlr.) halophila (formerly Ect. halophila) did not show any cross-hybridization even at low stringency. This result is in good accordance with a recently proposed reassignment within the family Ectothiorhodospiraceae, which included the separation of the extremely halophilic species into the new genus Halorhodospira. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
