Cloning and sequencing of the hrcA gene of Bacillus stearothermophilus |
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| Institution: | 1. Yunnan Key Laboratory for Palaeobiology, Yunnan University, Kunming 650091, China;2. MEC International Joint Laboratory for Palaeobiology and Palaeoenvironment, Yunnan University, Kunming 650091, China;3. School of Earth Sciences, University of Bristol, Wills Memorial Building, Queens Road, Bristol BS8 1RJ, UK;4. School of Biological Sciences, University of Bristol, Life Sciences, Building, 24 Tyndall Avenue, Bristol BS8 1TQ, UK;5. Department of Earth Sciences, The Natural History Museum, Cromwell Road, London SW7 5BD, UK;6. Palaeobiology Section, Department of Natural History, Royal Ontario Museum, Toronto, ON M5S 2C6, Canada;7. Yale Institute for Biosphere Studies, Yale University, New Haven, CT, USA;1. International Center for Research and Research Training in Endocrine Disruption of Male Reproduction and Child Health, Department of Growth and Reproduction, Rigshospitalet, University of Copenhagen, Copenhagen 2100, Denmark;2. Institute of Biomedicine, University of Turku and Department of Paediatrics, Turku University Hospital, Turku, Finland |
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| Abstract: | We report on cloning and sequencing of a 2.0-kb PCR fragment of chromosomal DNA from thermophilic Bacillus stearothermophilus carrying the complete hrcA gene. In addition, this amplicon contains the 3' end of an open reading frame exhibiting significant homology to the hemN gene of Bacillus subtilis (Bs) and other bacterial species. The hrcA gene could complement an Bs hrcA deletion mutant by repressing expression of class I heat shock (HS) genes. Furthermore, we could show that the HrcA protein derived from the thermophilic microorganism responds to HS in a similar way as reported for the Bs HrcA protein. |
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