Isolation of a full-length human WNT7A gene implicated in limb development and cell transformation,and mapping to chromosome 3p25 |
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| Affiliation: | 1. Imperial Cancer Research Fund, Institute of Molecular Medicine, University of Oxford, John Radcliffe Hospital, Headington, Oxford OX3 9DU, UK;2. University of Newcastle upon Tyne, Molecular Genetics Unit, Ridley Building, Newcastle upon Tyne NE1 7RU, UK;1. Randall Division of Cell and Molecular Biophysics, Guy’s Campus, King’s College London, London SE1 1UL, UK;2. Procedural Services Section, MRC-National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA, UK;1. Institut de la Main, Clinique Bizet, 22, rue Georges-Bizet, 75116 Paris, France;2. Hôpital Necker-Enfants–Malades, 149, rue de Sèvres, 75015 Paris, France;1. Institute of Zoology and Center of Molecular Bioscience Innsbruck, University of Innsbruck, Technikerstr. 25, A-6020 Innsbruck, Austria;2. Department of Zoology, University of Oxford, South Parks Road, Oxford OX1 3PS, United Kingdom;3. Division of Physiology, Medical University of Innsbruck, Schöpfstraße 41/EG, A-6020 Innsbruck, Austria;4. Department of Biosystems Science and Engineering, ETH Zürich, Mattenstrasse 26, 4058 Basel, Switzerland;5. European Research Institute for the Biology of Ageing, University of Groningen, University Medical Center Groningen, A. Deusinglaan 1, NL-9713 AV Groningen, The Netherlands |
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| Abstract: | The Wnt gene family has a role in development as well as tumourigenesis. One mouse member, Wnt7a, is vital for limb development in vivo and also possesses transforming ability in vitro. This study reports the isolation of a full length of human homologue of mouse Wnt7a gene by library screening. Yeast artificial chromosome-fluorescence in situ hybridisation (YAC-FISH) mapped the WNT7A gene to chromosome 3p25. Human WNT7A had an ORF encoding a deduced protein of 349 aa that exhibited 97% and 92% identity to mouse Wnt7a at the aa and nucleic acid levels, respectively. It possessed the 22 conserved cysteine residues and 3 more at the amino terminus, and a putative poly A tail. This is the fifth human WNT gene in which a complete cDNA sequence had been determined. |
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