Shigella flexneri type-specific antigen V: cloning,sequencing and characterization of the glucosyl transferase gene of temperate bacteriophage SfV |
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| Institution: | 1. Department of Cell and Systems Biology, University of Toronto, 25 Willcocks Street, Toronto, Ontario, Canada M5S 3B2;2. Department of Molecular Genetics, University of Toronto, 1 King''s College Cir, Toronto, Ontario, Canada M5S 1A8;3. Centre for the Analysis of Genome Evolution and Function, University of Toronto, Toronto, Ontario, Canada M5S 3B2;1. Forzani & MacPhail Colon Cancer Screening Centre, University of Calgary, Calgary, AB, Canada;2. Department of Cancer Epidemiology and Prevention Research, Cancer Control Alberta, Alberta Health Services, Calgary, AB, Canada;3. Department of Oncology, Cumming School of Medicine, University of Calgary, Calgary, AB, Canada;4. Department of Medicine, Cumming School of Medicine, University of Calgary, Calgary, AB, Canada;5. Department of Community Health Sciences, Cumming School of Medicine, University of Calgary, Calgary, AB, Canada |
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| Abstract: | With lysogeny by bacteriophage SfV, Shigella flexneri serotype Y is converted to serotype 5a. The glucosyl transferase gene (gtr) from bacteriophage SfV of S. flexneri, involved in serotype-specific conversion, was cloned and characterized. The DNA sequence of a 3.7 kb EcoRI–BamHI fragment of bacteriophage SfV which includes the gtr gene was determined. This gene, encoding a polypeptide of 417 aa with 47.67 kDa molecular mass, caused partial serotype conversion of S. flexneri from serotype Y to type V antigen as demonstrated by Western blotting and the sensitivity of the hybrid strain to phage Sf6. The deduced protein of the partially sequenced open reading frame upstream of the gtr showed similarity to various glycosyl transferases of other bacteria. Orf3, separated from the gtr by a non-coding region and transcribed convergently, codes for a 167 aa (18.8 kDa) protein found to have homology with tail fibre genes of phage lambda and P2. |
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