Structural properties and RNA-binding activities of two RNA recognition motifs of a mouse neural RNA-binding protein,mouse-Musashi-1 |
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| Affiliation: | 1. Department of Bioengineering, Faculty of Engineering, Yokohama National University, Yokohama 230 Japan;2. Department of Molecular Neurobiology, Institute of Basic Medical Sciences, University of Tsukuba, Tsukuba, Ibaraki 305 Japan;3. Center for TARA (Okano Project), University of Tsukuba, Tsukuba, Ibaraki 305 Japan;4. CREST, Japan Science and Technology Corporation (JST), Shinagawa, Tokyo 140 Japan;5. Biomedical Research Center, Osaka University Medical School, Suita, Osaka 565 Japan;1. Department of Biology, Centre for Vision Research, York University, 4700 Keele Street, Toronto, Ontario M6J 1P3, Canada;2. Department of Physiology and Pharmacology, Centre for Functional and Metabolic Mapping, University of Western Ontario, 1151 Richmond Street North, Ontario N6A 5B7, Canada;1. Heart Center, Turku University Hospital and University of Turku, Turku, Finland;2. Research Center of Applied and Preventive Cardiovascular Medicine, University of Turku, Turku, Finland;3. Division of Perioperative Services, Intensive Care Medicine and Pain Management, Turku University Hospital and University of Turku, Turku, Finland;4. Department of Neurology, North Karelia Central Hospital, Siun Sote, Joensuu, Finland;5. Department of Neurology, University of Turku, Turku, Finland;6. National Institute for Health and Welfare, Helsinki, Finland;7. Division of Medicine, University of Turku and Turku University Hospital, Turku, Finland;8. Department of Public Health, University of Turku, Turku, Finland;9. Turku Clinical Research Centre, Turku University Hospital, Turku, Finland;1. Genetics-Biotechnology Center, University of Wisconsin-Madison School of Medicine and Public Health, USA;2. Department of Chemical and Biological Engineering, University of Wisconsin-Madison College of Engineering, USA;3. Microbiology Doctoral Training Program, University of Wisconsin-Madison, USA;1. Imperial College Centre for Synthetic Biology, Imperial College London, SW7 2AZ, London, UK;2. Department of Bioengineering, Imperial College London, SW7 2AZ, London, UK;1. Spemann Graduate School of Biology and Medicine (SGBM), Albert Ludwigs University of Freiburg, Freiburg, Germany;2. Centers for Biological Signalling Studies BIOSS and CIBSS, Albert Ludwigs University of Freiburg, Freiburg, Germany;3. Faculty of Biology, Institute of Biology II, Albert Ludwigs University of Freiburg, Freiburg, Germany |
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| Abstract: | mouse-Musashi-1 (m-Msi-1) is an RNA-binding protein, abundantly expressed in the developing mammalian central nervous system (CNS). m-Msi-1 contains two RNA recognition motifs (RRMs). In this study, we found that the N-terminal RRM of m-Msi-1 (MMA) binds strongly to poly(G) and weakly to poly(U) in a way similar to that of the full-length m-Msi-1 protein characterized previously. The C-terminal RRM of m-Msi-1 (MMB), however, does not bind to RNA. In addition, the circular dichroism (CD) spectra of the two RRMs showed that the α-helical content of MMA is significantly higher than that of MMB, indicating that some differences in the secondary structure may be responsible for the distinct RNA binding properties of MMA and MMB. |
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