Cloning of the cDNA encoding rat homologue of the mismatch repair gene MSH2 and its expression during spermatogenesis |
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| Institution: | 1. Department of Obstetrics and Gynecology, Southwest Hospital, Third Military Medical University (Army Medical University), Chongqing 400038, PR China;2. Department of Transfusion Medicine, Southwest Hospital, Third Military Medical University (Army Medical University), Chongqing 400038, PR China;3. Department of Laboratory Medicine, Southwest Hospital, Third Military Medical University (Army Medical University), Chongqing 400038, PR China;1. Biozentrum, University Hospital Basel, Switzerland;2. Clinical Microbiology, University Hospital Basel, Switzerland;3. Applied Microbiology Research, Department of Biomedicine, University of Basel, CH-4056 Basel, Switzerland;4. BioVersys AG, Hochbergerstrasse 60C, Technology Park, 4057 Basel, Switzerland |
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| Abstract: | A rat cDNA clone encoding the mismatch repair protein MSH2 has been isolated and characterized. The cDNA has an open reading frame of 2802 nucleotides in length coding for a protein of 933 amino acids (100 kDa). It shows significant homology to human and mouse MSH2. Northern blot analysis of rat MSH2 in the testes of rats of different ages showed maximum expression at 20 days, at which time the germ cells are undergoing premeiotic DNA replication. We observed down-regulation in the expression of rat MSH2 beyond 25 days by which time the germ cells have entered meiotic prophase. |
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