|
|||||
|
|
Separation of coeliac-active peptides from bread wheat with the aid of methylpyrrolidinone chitosan |
|
| Authors: | M De Vincenzi M R Dessì R A A Muzzarelli |
| Institution: | Department of Metabolism and Pathological Biochemistry, Istituto Superiore di Sanità, Viale Regina Elena 299, IT-00161, Roma, Italy Institute of Biochemistry, Faculty of Pharmacy, University of Cagliari, Viale della Pineta 77, IT-00910, Cagliari, Italy Institute of Biochemistry, Faculty of Medicine, University of Ancona, Via Ranieri 67, IT-60100, Ancona, Italy |
| Abstract: | Gliadin-derived peptides from haxaploid wheat, known to be toxic for in-vitro cultured small-intestinal mucosa from coeliac patients, are very active in agglutinating K562(S) cells. Chromatographic data obtained with the use of methylpyrrolidinone chitosan coupled to Sepharose-6B indicate that a minor (1·3%) fraction of the peptides binds very strongly to the support at nearly neutral pH values, and can be eluted at pH 2·85. This fraction is the one responsible for the agglutination of K562(S) cells, being one-hundred times more active than the digest from which it has been separated. |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ | |