Adsorptive immobilization of rhodococcal cells in hydrophobized derivatives of wide-pore poly(acrylamide) cryogel |
| |
Authors: | M S Kuyukina I B Ivshina E V Rubtsova R V Ivanov V I Lozinsky |
| |
Institution: | 1.Institute of Ecology and Genetics of Microorganisms, Ural Branch,Russian Academy of Sciences,Perm,Russia;2.Nesmeyanov Institute of Organoelement Compounds,Russian Academy of Sciences,Moscow,Russia |
| |
Abstract: | Adsorption of Rhodococcus ruber cells on columns with poly(acrylamide) cryogel (cryoPAAG) partially hydrophobized by different quantities (0.2, 1, and 5, mol %) of chemically grafted n-dodecane residues has been
studied. The adsorption capacity (1.1 × 109 cells/g) of gel carrier for rhodococcal cells and the optimal content (1 mol %) of hydrophobizing groups were determined.
The respirometric method showed the high catalytic activity and functional stability of immobilized bacterial cells. Respiratory
activity of immobilized rhodococci in the presence of a model mixture of oil hydrocarbons exceeded the respective parameter
for free cells by 12–17%. Viability of rhodococcal cells adsorptionally fixed in hydrophobized cryoPAAG was maintained at
a level of 93–95% after a half-year period of storage. The results may be used for development of immobilized biocatalyst
for directed transformation of hydrocarbon compounds and biological purification of oil-polluted water. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|