Identification,characterisation and mapping of simple sequence repeat (SSR) markers from raspberry root and bud ESTs |
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Authors: | M Woodhead S McCallum K Smith L Cardle L Mazzitelli J Graham |
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Institution: | (1) Scottish Crop Research Institute, Invergowrie, Dundee, DD2 5DA, UK |
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Abstract: | Raspberry breeding is a long, slow process in this highly heterozygous out-breeder. Selections for complex traits like fruit
quality are broad-based and few simple methodologies and resources are available for glasshouse and field screening for key
pest and disease resistances. Additionally, the timescale for selection of favourable agronomic traits requires data from
different seasons and environmental locations before any breeder selection can proceed to finished cultivar. Genetic linkage
mapping offers the possibility of a more knowledge-based approach to breeding through linking favourable traits to markers
and candidate genes on genetic linkage maps. To further increase the usefulness of existing maps, a set of 25 polymorphic
SSRs derived from expressed sequences (EST-SSRs) have been developed in red raspberry (Rubus idaeus). Two different types of expressed sequences were targeted. One type was derived from a root cDNA library as a first step
in assessing sequences which may be involved in root vigour and root rot disease resistance and the second type were ESTs
from a gene discovery project examining bud dormancy release and seasonality. The SSRs detect between 2 and 4 alleles per
locus and were assigned to linkage groups on the existing ‘Glen Moy’ × ‘Latham’ map following genotyping of 188 progeny and
examined for association with previously mapped QTL. The loci were also tested on a diverse range of Rubus species to determine transferability and usefulness for germplasm diversity studies and the introgression of favourable alleles. |
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Keywords: | Expressed sequence tags Rubus idaeus Simple sequence repeats Raspberry EST-SSRs QTL |
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