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Improved product formation in high density Chinese hamster ovary cell cultures transfected at confluency |
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| Authors: | Thomas Noll Detlef Eisenkrätzer André Kiesewetter André Dinter Steffen Zeng Christian Wandrey Manfred Biselli Eric G. Berger |
| Affiliation: | (1) Institute of Biotechnology, Research Center Jülich, Jülich, Germany;(2) Pharma Biotech Development & Production, Animal Cell Fermentation, Bldg. 352/563, Roche Diagnostics GmbH, Nonnenwald 2, D-82377 Penzberg, Germany;(3) Institute of Physiology, University of Zürich, Zürich, Switzerland;(4) Biochemie GmbH, R&D – Bioproducts, Cell Culture Process Development, Biochemiestrasse 10, A-6250 Kundl/Tirol, Austria;(5) University of Applied Sciences Aachen, Ginsterweg 1, D-52428 Jülich, Germany |
| Abstract: | Cell-specific productivity was compared in Chinese hamster ovary cell clones transfected at confluency versus subconfluency. Following lipofection of a pCDNA1-derived vector encoding a proteinA-fucosyltransferaseIV fusion protein, selection and single cell sorting by flow cytometry, clones were expanded to tissue flasks and assessed for cell specific productivity. The probability of obtaining high producers at confluency was significantly higher from cells transfected at confluency than at subconfluency (P=0.05). |
| Keywords: | cell cycle cell-specific productivity fluidized bed reactor fucosyltransferase IV |
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