Effect of modified carbon allocation on turgor, osmolality, sugar and potassium content, and membrane potential in the epidermis of transgenic potato (Solanum tuberosum L.) plants |
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Authors: | Kehr J; Wagner C; Willmitzer L; Fisahn J |
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Institution: | Max-Planck-Institut fur Molekulare Pflanzenphysiologie, Karl-Liebknecht-Strasse 25, 14476 Golm, Germany; Corresponding author; Fax: +49 331 977 2301; E-mail: fisahn@mpimp-golm.mpg.de |
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Abstract: | The effects of modification in sugar concentrations on turgor pressure and
membrane potential in epidermal leaf cells of transgenic potato
(Solanum tuberosum cv. Desirée) plants were
studied. Measurements of turgor pressure were performed by insertion of a
micro pressure probe. Osmolality and sugar concentrations were determined
by micro analysis of single cell extracts. Membrane potentials and cell
diameters were calculated from repeated, computer-controlled scans with
voltage-sensitive microelectrodes. Epidermal cells of sucrose transporter
antisense plants showed a more than 100% elevation in osmolality and turgor
pressure compared to the wild-type. As a consequence, cell diameters were
enhanced in this transgenic line. However, membrane potentials were only
slightly reduced in sucrose transporter antisense plants. In addition to
sucrose transporter antisense lines, transgenic plants that were reduced in
their capacity to accumulate starch due to antisense inhibition of the
chloroplastic fructose-1,6-bisphosphatase (FBPase) were investigated. These
antisense plants maintained membrane potential and turgor pressure
comparable to the wild-type. |
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