Global gene expression profiling of Homarus americanus (Crustacea) larval stages during development and metamorphosis |
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| Authors: | Daniel J Hines K Fraser Clark Spencer J Greenwood |
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| Institution: | 1. AVC Lobster Science Centre, Atlantic Veterinary College, University of Prince Edward Island, Charlottetown, PE, C1A 4P3, Canada.;2. Department of Pathology and Microbiology, Atlantic Veterinary College, University of Prince Edward Island, Charlottetown, PE, C1A 4P3, Canada.;3. Department of Biomedical Sciences, Atlantic Veterinary College, University of Prince Edward Island, Charlottetown, PE, C1A 4P3, Canada. |
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| Abstract: | Homarus americanus has a life history that is similar to other arthropods, including a pelagic larval phase and a benthic adult phase. The larval phase is divided into three morphologically distinct stages, followed by metamorphosis to the post-larval phase. H. americanus larval development has been studied previously, although the molecular mechanisms that regulate the consequent changes are not fully elucidated. This study is the first to use an oligonucleotide microarray to investigate global gene expression during H. americanus larval development. Stage-specific gene expression profiles of larvae and postlarvae from two-year classes were assessed. We found the expression levels of 1851 genes to be significantly different among larval stages. Functional annotations indicated that various differentially expressed genes were involved with immune function, energy regulation, and development. Ten target genes of interest were selected for expression verification using RT-qPCR. Two Phosphoenolpyruvatecarboxykinases, Argonaute 2, Ecdysone-inducible protein 75, and Procollagen-lysine 2-oxoglutarate 5-dioxygenase 3, had significantly different expression (p?
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| Keywords: | microarray RT-qPCR Decapoda lobster |
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