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Barnacle cyprid motility and distribution in the water column as an indicator of the settlement-inhibiting potential of nontoxic antifouling chemistries
Authors:Stojan Maleschlijski  Stella Bauer  Alessio Di Fino  G Hernan Sendra  Anthony S Clare  Axel Rosenhahn
Institution:1. Institute of Functional Interfaces, Karlsruhe Institute of Technology, Karlsruhe, Germany;2. Applied Physical Chemistry, University of Heidelberg, Heidelberg, Germany;3. Analytical Chemistry – Biointerfaces, Ruhr-University Bochum, Bochum, Germany;4. School of Marine Science and Technology, Newcastle University, Newcastle-upon-Tyne, UK;5. Applied Physical Chemistry, University of Heidelberg, Heidelberg, Germany;6. Analytical Chemistry – Biointerfaces, Ruhr-University Bochum, Bochum, Germany
Abstract:Testing of new coatings to control fouling frequently involves single-species laboratory bioassays. Barnacle cyprids are among the most widely used model organisms in marine biofouling research, and surfaces that inhibit their settlement are considered to be promising candidates for new coating concepts. An analysis of motility parameters (mean velocity and swimming area coefficient) and distribution of cyprids of Balanus amphitrite in different swimming regions in the vicinity of model surfaces (self-assembled monolayers) is presented. The data are correlated with the settlement preferences of cyprids on these surfaces. Cyprids were predominantly found in interfacial regions and the transition frequencies between swimming regions of different depths were determined.
Keywords:cyprid  antifouling  settlement assay  3D tracking  motility  swimming regions
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