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The nucleotide sequence of the lipo-penicillinase gene of alkalophilic Bacillus sp. strain 170 |
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| Authors: | Chiako Kato Yoshio Nakano Koki Horikoshi |
| Affiliation: | (1) Laboratory of Microbiol Metabolism, The Superbugs Project, Research Development Corporation of Japan, 2-28-8 Honkomagome, Bunkyo-ku, Tokyo 113, Japan;(2) Present address: Central Research Laboratory, ZERIA Pharmaceutical Co. Ltd., Osato, 360-01 Saitama, Japan;(3) Present address: Laboratory of Biochemistry, College of Science, Rikkyo University, Nishi-Ikebukuro, Toshima-ku, Tokyo 117, Japan;(4) Present address: Department of Bio-Engineering, Tokyo Institute of Technology, O-okayama, Meguro-ku, Tokyo 152, Japan |
| Abstract: | The lipo-penicillinase (LIPEN) gene from an alkalophilic Bacillus sp. strain 170 was cloned in Escherichia coli using the vector pHSG399. A plasmid, pFAP121, was isolated from an ampicillin resistant transformant and the cloned LIPEN gene was found to be in a 2.2 kb DNA fragment. The nucleotide sequence of a 1.9 kb segment encoding the LIPEN was determined. This segment showed an open reading frame which would encode a polypeptide of 310 amino acids. The amino acid sequence of this LIPEN gene product has strong homology with those of the Bacillus cereus  -lactamase III and Bacillus licheniformis penicillinase. |
| Keywords: | Alkalophilic Bacillus Penicillinase |
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