Expression of NMDA Receptor and its Effect on Cell Proliferation in the Subventricular Zone of Neonatal Rat Brain |
| |
Authors: | Hongbin Fan Junying Gao Wei Wang Xiaoquan Li Teijun Xu Xiaoxing Yin |
| |
Institution: | (1) Jiangsu Key Laboratory of Neurodegeneration, Department of Pharmacology, Nanjing Medical University, Nanjing, 210029, China;(2) Department of Neurology, Affiliated Hospital of Xuzhou Medical College, Xuzhou, 221002, China;(3) Department of Anatomy, Nanjing Medical University, Nanjing, 210029, China;(4) Department of Anatomy and Neurobiology, Xuzhou Medical College, 84 West Huaihai Road, Xuzhou, 221004, Jiangsu, China;(5) Department of Clinical Pharmacology, School of Pharmacy, Xuzhou Medical College, 84 West Huaihai Road, Xuzhou, 221004, Jiangsu, China; |
| |
Abstract: | We investigated the involvement of N-methyl d-aspartate receptor (NMDAR) in neurogenesis of rat’s subventricular zone (SVZ). For this purpose, we determined expression
of the NMDAR subunits NR1, NR2A, and NR2B in SVZ of the neonatal Sprague-Dawley rats using immunohistochemical techniques.
All three NMDAR subunits were expressed during postnatal day (PND)-1 to PND-28 whereas each subunit showed a distinct expression
pattern. We also examined the functional effect of this receptor on cell proliferation in this region and, in this regard,
the animals received either intraperitoneal injection of NMDAR agonist NMDA (2 mg/kg/day) or selective non-competitive NMDAR
antagonist MK-801 (10 mg/kg) or NR2B antagonist Ro25-6981 (40 mg/kg), respectively, at PND-3. A significant developmental
increase of the total cell density was observed at PND-7 (P < 0.05) while proliferating cell nuclear antigen-positive cell density was significantly increased at PND-14 (P < 0.05) and at PND-28 (P < 0.05) in the SVZ after NMDA (2 mg/kg/day) injection. Our data show that the NMDAR activation promoted the cell proliferation
in SVZ during the neonatal period. We, therefore, inferred that NMDAR is expressed in SVZ of the neonatal rat brain and can
promote neurogenesis, as through cell proliferation process in that region, and can thus be used as a potential therapeutic
target in neurodegenerative diseases. |
| |
Keywords: | |
本文献已被 PubMed SpringerLink 等数据库收录! |
|