Chromosomal location of a gene (idg) that specifies production of the blue pigment indigoidine inErwinia chrysanthemi |
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Authors: | Arun K Chatterjee Mary Ann Brown |
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Institution: | (1) Department of Plant Pathology, Kansas State University, 66506 Manhattan, Kansas, USA |
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Abstract: | Production of a blue pigment, indigoidine, is a variable trait among wild-type strains ofErwinia chrysanthemi; it is also influenced by the composition of the growth medium. Starting with a nonpigmented wild-type strain (ICPB EC183) ofE. chrysanthemi, we obtained by nitrosoguanidine mutagenesis a pigmented (idg
+) mutant strain (AC6055), which simultaneously was Arg–. Linkage betweenarg andidg was established in two-factor transductional and conjugational crosses. Coinheritance ofidg withhis
+,ilv
+,leu
+,ser
+,thr
+, orura
+ transductants was not observed. Spontaneous Arg+ revertants of AC6055 were invariably Idg+. The pigments produced by AC6055, Idg+ recombinants, and wild-type Idg+ strains were identical, judged by absorption spectra ( max = 615 nm) of the dimethylsulfoxide extracts of whole cells. We concluded that nitrosoguanidine caused comutation in thearg andidg loci that are linked on theE. chrysanthemi chromosome. |
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Keywords: | |
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