Physiology and proteomics of the water-deficit stress response in three contrasting peanut genotypes |
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Authors: | KAMESWARA RAO KOTTAPALLI RANDEEP RAKWAL JUNKO SHIBATO GLORIA BUROW DAVID TISSUE JOHN BURKE NAVEEN PUPPALA MARK BUROW & PAXTON PAYTON |
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Institution: | Departments of Plant and Soil Science and;Biology, Texas Tech University, Lubbock, TX 79409, USA,;United States Department of Agriculture Cropping Systems Research Laboratory, Lubbock, TX 79415, USA,;Health Technology Research Center (HTRC), National Institute of Advanced Industrial Science and Technology WEST, Onogawa 16-1, Tsukuba, 305-8569, Japan,;Research Laboratory for Biotechnology and Biochemistry (RLABB), Kathmandu, Nepal,;University of Western Sydney, Centre for Plant and Food Science, Richmond, NSW 2753, Australia,;New Mexico State University Agricultural Science Center, Clovis, NM 88101, USA and;Texas Agrilife Research and Extension Center Texas A&M System, Lubbock, TX 79403, USA |
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Abstract: | Peanut genotypes from the US mini-core collection were analysed for changes in leaf proteins during reproductive stage growth under water-deficit stress. One- and two-dimensional gel electrophoresis (1- and 2-DGE) was performed on soluble protein extracts of selected tolerant and susceptible genotypes. A total of 102 protein bands/spots were analysed by matrix-assisted laser desorption/ionization–time-of-flight mass spectrometry (MALDI–TOF MS) and by quadrupole time-of-flight tandem mass spectrometry (Q-TOF MS/MS) analysis. Forty-nine non-redundant proteins were identified, implicating a variety of stress response mechanisms in peanut. Lipoxygenase and 1 l -myo-inositol-1-phosphate synthase, which aid in inter- and intracellular stress signalling, were more abundant in tolerant genotypes under water-deficit stress. Acetyl-CoA carboxylase, a key enzyme of lipid biosynthesis, increased in relative abundance along with a corresponding increase in epicuticular wax content in the tolerant genotype, suggesting an additional mechanism for water conservation and stress tolerance. Additionally, there was a marked decrease in the abundance of several photosynthetic proteins in the tolerant genotype, along with a concomitant decrease in net photosynthesis in response to water-deficit stress. Differential regulation of leaf proteins involved in a variety of cellular functions (e.g. cell wall strengthening, signal transduction, energy metabolism, cellular detoxification and gene regulation) indicates that these molecules could affect the molecular mechanism of water-deficit stress tolerance in peanut. |
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Keywords: | 1-DGE 2-DGE peanut mini-core drought |
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