A METHOD FOR THE DETERMINATION OF DIFFUSION CONSTANTS AND THE CALCULATION OF THE RADIUS AND WEIGHT OF THE HEMOGLOBIN MOLECULE |
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| Authors: | John H. Northrop M. L. Anson |
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| Affiliation: | From the Laboratories of The Rockefeller Institute for Medical Research, Princeton, N. J. |
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| Abstract: | A method is described for determining the diffusion coefficient of solutes by determining the rate of passage of the solute through a thin porous membrane between two solutions of different concentration. The method has been used to determine the diffusion coefficient of carbon monoxide hemoglobin. This was found to be 0.0420 ± 0.0005 cm.2 per day at 5°C. The molecular weight of carbon monoxide hemoglobin calculated by means of Einstein''s equation from this quantity is 68,600 ± 1,000. |
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