Characterization of the folate receptor in human molar placenta |
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Authors: | Jan Holm Steen Ingemann Hansen Carl W Nichols Mimi Høier-Madsen Poul Erik Helkjær |
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Institution: | (1) Department of Clinical Chemistry, Horsens Hospital, DK-8700 Horsens, Denmark;(2) Department of Clinical Chemistry, Central Hospital, DK-3400 Hillerød, Denmark;(3) Department of Pathology, West Paces Medical Center, 3200 Howell Mill Rd., 30327 NW Atlanta, Georgia, USA;(4) Laboratory for Autoimmune Serology, State Serum Institute, DK-2300 S Copenhagen, Denmark;(5) Department of Obstetrics and Gynaecology, Central Hospital, DK-4700 Næstved, Denmark |
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Abstract: | We have characterized a high-affinity folate receptor in human molar placenta tissue. Radioligand binding exhibited characteristics typical of other high-affinity folate binding proteins. Those included, positive cooperativity, a tendency to increased binding affinity with decreasing receptor concentration, a slow ligand dissociation at pH 7.4 becoming rapid at pH 3.5, and inhibition by folate analogues. The folate receptor cross-reacted with antibodies against human milk folate binding protein, e.g. the syncytothrophoblastic layer of molar placenta tissue sections showed strongly positive immunostaining. The gel filtration profile contained two radioligand-bound peaks (25 and 100 kDa), however, with considerable overlap. Only a single band of 70 kDa was seen on SDS-PAGE immunoblotting. The folate receptor in placental tissue may play a crucial role in the transfer of folate from maternal circulation to the fetus. |
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Keywords: | Folate receptor mola placenta |
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