A method for separation of pigments from plasmodia of the true slime molds, Physarum polycephalum and Physarum nudum |
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| Authors: | A Majcherczyk L Rakoczy A Hüttermann |
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| Affiliation: | 1. Department of Applied Chemistry and Chemical Engineering, Faculty of Engineering and Technology, University of Dhaka, Dhaka, Bangladesh;2. Department of Chemical Engineering and Polymer Science, Shahjalal University of Science and Technology, Sylhet, Bangladesh;3. National Institute of Textile Engineering and Research (NITER), Dhaka, Bangladesh;4. Department of Arts and Sciences, Ahsanullah University of Science and Technology, Dhaka, Bangladesh;1. Canadian Cancer Trials Group, Queen’s University, Kingston, ON, Canada;2. Division of Cancer Care and Epidemiology, Cancer Research Institute, Queen’s University, Kingston, ON, Canada;3. Department of Oncology, Queen’s University, Kingston, ON, Canada;4. Department of Surgery, Queen’s University, Kingston, ON, Canada;5. Department of Public Health Sciences, Queen’s University, Kingston, ON, Canada;1. Centre for HIV and Sexually Transmitted Infections, National Institute for Communicable Diseases, National Health Laboratory Service, Johannesburg, South Africa;2. Department of Clinical Microbiology & Infectious Diseases, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, South Africa;1. Division of Hematology and Oncology, Mayo Clinic, Jacksonville, FL;2. Division of Biomedical Statistics and Informatics, Mayo Clinic, Jacksonville, FL;3. Division of Colon and Rectal Surgery, Mayo Clinic, Jacksonville, FL;1. School of Bioengineering, Dalian University of Technology, Dalian, 116024, China;2. Ministry of Education Center for Food Safety of Animal Origin, Dalian 116600, China;3. Shanghai Public Health Clinical Center, Fudan University, Shanghai 200000, China |
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| Abstract: | Plasmodia of the true slime molds Physarum polycephalum and Physarum nudum were grown on agar semidefined medium (J. W. Daniel and H. Baldwin (1964) in Methods in Cell Physiology (Precsott, D. M., Ed.), Vol. 1, pp. 9-44, Academic Press, New York.) and pigments were extracted from 8-day-old plasmodia with the same solvent mixture that was used later as a mobile phase in the HPLC analysis. The separation was carried out on a strong anion exchanger column in a methanol/borate buffer solution. Under these conditions eight pigments from P. polycephalum and eight from P. nudum were revealed. This method can be used for detailed studies of plasmodial pigments in these species. |
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