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Improvement in Yield and Purity of a Recombinant Malaria Vaccine Candidate Based on the Receptor-Binding Domain of Plasmodium vivax Duffy Binding Protein by Codon Optimization |
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| Authors: | Syed Shams Yazdani Ahmad Rushdi Shakri Priyabrata Pattnaik M. Moshahid A. Rizvi Chetan E. Chitnis |
| Affiliation: | (1) Malaria Research Group, International Centre for Genetic Engineering and Biotechnology (ICGEB), Aruna Asaf Ali Marg, New Delhi, 110067, India;(2) Defence Research & Development Establishment, Jhansi Road, Gwalior, 474 002, India;(3) Department of Biosciences, Jamia Millia Islamia, Jamia Nagar, New Delhi, 110025, India |
| Abstract: | A recombinant blood-stage vaccine for Plasmodium vivax malaria based on the functional receptor-binding domain of PvDBP (PvRII) has been developed. A synthetic gene coding for PvRII was expressed in Escherichia coli using codon optimization. Expression level of recombinant PvRII was 10% of the total cellular proteins. Truncated PvRII products, seen when the native PvRII gene was expressed, were absent in case of synthetic gene. |
| Keywords: | Codon optimization Duffy binding protein Malaria vaccine Recombinant Escherichia coli Synthetic gene |
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