Expression of a gene (Ids2) induced by Fe deficiency treatment in the roots of Hordeum vulgare L. |
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| Authors: | Mori S Okumura N Nakanishi H Umehara Y Chino M Nishizawa N K |
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| Institution: | (1) Department of Agricultural Chemistry, The University of Tokyo, 113 Tokyo, Japan |
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| Abstract: | A  ZAPII-cDNA library constructed from poly(A)+-RNA isolated from Fe-deficient barley roots was used for differential screening of barley roots grown in the presence and absence of Fe. Among seven clones that hybridised specifically to the probe for Fe deficiency, one clone (Ids2) was sequenced. Using a part of the cDNA sequence as a probe, a genomic-DNA library was probed and a corresponding DNA clone was isolated and sequenced. The predicted amino acid sequence resembled 2-oxoglutarate-dependent dioxygenase. Ids2 had a metal regulatory element as well as Cu regulatory elements of CUP1 gene of yeast MT. |
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| Keywords: | Barley root cDNA dioxygenase Ids2 iron deficiency mugineic acid |
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