A new type of muconate cycloisomerase from Rhodococcus rhodochrous strain 89 |
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Authors: | Solyanikova I P Schlömann M Golovleva L A |
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Institution: | (1) Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Pushchino, Moscow Region, 142290, Russia;(2) Interdisciplinary Ecological Center, Freiberg University of Mining and Technology, Leipziger str. 29, D-09599 Freiberg, Germany |
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Abstract: | Muconate cycloisomerase (MCI) was purified from Rhodococcus rhodochrous 89 grown on phenol. The enzyme appears to contain two different type subunits with molecular masses 35.5 and 37 kD. The N-terminal amino acid sequence of both subunits showed more similarity to corresponding enzymes from gram-negative bacteria than to one from Rhodococcus opacus 1CP. MCI from R. rhodochrous 89, like analogous enzymes from gram-negative bacteria, can convert 2-chloromuconate (2-CM) with the formation of both, 2- and 5-chloromuconolactones (CML) as intermediates. Nevertheless, its unique ability to convert 5-CML to cis- but not to trans-dienelactone sets it apart from all known chloromuconate cycloisomerases from gram-negative and gram-positive bacteria. |
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Keywords: | (chloro)muconate cycloisomerase transformation 2-chloromuconate chloromuconolactones dienelactones Rhodococcus rhodochrous 89 |
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