Vergleichende Untersuchungen an HNO2-Inaktivierten und UV-Inaktivierten Bakteriophagen T4 |
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| Authors: | Walter Harm |
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| Institution: | (1) Abteilung Strahlenbiologie, Institut für Genetik der Universität Köln, Gyrhofstr. 15-17, Köln-Lindenthal |
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| Abstract: | Summary The inactivation of phage T4 by nitrous acid (HNO2) is essentially an exponential function of time of treatment. HNO2-inactivated T4 is able to undergo multiplicity reactivation, and genetic markers may be rescued by live phage, however, the extent of both effects is appreciably less than after UV-inactivation. Also, the survival of phenotypic function of the cistronsr II-A andr II-B is lower with HNO2-treatment than with a UV-irradiation of a corresponding number of hits.The reduced effects are quantitatively accounted for by the assumption of lethal hits blocking early steps of infection. These early-step damages amount to approximately 1/6 of the total hit number; it is still unknown whether they occur in DNA or in protein. Some indication for the occurrence in protein comes from the result that the host-killing efficiency of HNO2-inactivated phage is reduced at a similar rate as these early-step damages occur. However, at least 5/6 of the lethal hits are due to chemical changes within the DNA, as can be calculated from the results of multiplicity reactivation, marker rescue, and phenotypic survival of therII-cistrons.
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