| 我国辽宁，吉林地区恙虫病东方体分离株的PCR分型 |
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| 引用本文： | 张志强,胡玲美,鲁志新,冯立,杨青,吴益民.我国辽宁，吉林地区恙虫病东方体分离株的PCR分型[J].微生物学杂志,2001,21(1):29-30. |
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| 作者姓名： | 张志强 胡玲美 鲁志新 冯立 杨青 吴益民 |
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| 作者单位： | 沈阳军区联勤部军事医学研究所, |
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| 摘 要： | 为鉴定我国东北地区恙虫病东方体(Ot)型别,本文应用Ot外膜主要蛋白型特异抗原56ku构建的群、型引物,采用PCR技术对分离自辽宁、吉林地区的6株恙虫病东方体目的基因进行分析研究,并与Gilliam,Karp,Kato国际参考株进行比较.结果表明,辽宁、吉林恙虫病东方体分离株至少存在Gilliam和Karp2种型别.
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| 关 键 词： | 聚合酶链反应(PCR) 恙虫病东方体 基因型分析 |
| 文章编号： | 1005-7021(2001)01-0029-02 |
| 修稿时间： | 2000年8月11日 |
Gene Typing of Orientia tsutsugamushi Strain Isolated from Liaoning and Jilin by PCR |
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| Zhang Zhiqiang et al..Gene Typing of Orientia tsutsugamushi Strain Isolated from Liaoning and Jilin by PCR[J].Journal of Microbiology,2001,21(1):29-30. |
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| Authors: | Zhang Zhiqiang |
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| Abstract: | In order to identify the types of Orientia tsutsugamushi (Ot ), 56ku of main protein peculiar antigen from outer membrane of Ot was used as type and type specific primers. Target genes from six Ot strains isolated from Liaoning and Jilin were analyzed by PCR, and comparisons were made with Gilliam, Karp, and Kato international reference strains. The results showed that at least two types exist in Ot from Liaoning and Jilin i.e. Gilliam and Karp. |
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| Keywords: | PCR Orientia tsutsugamushi (Ot ) gene type |
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