Inulase-secreting strain of Saccharomyces cerevisiae produces fructose |
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Authors: | Brevnova E E Kozlov D G Efremov B D Benevolensky S V |
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Affiliation: | Institute of Genetics and Selection of Industrial Microorganisms, Moscow 113545, Russia. |
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Abstract: | The gene encoding inulase of the yeast Kluyveromyces marxianus (INU1Km) was cloned and expressed in the inulin-negative yeast Saccharomyces cerevisiae. Cells of S. cerevisiae transformed with the INU1Km gene have acquired extracellular inulase activity and were able to grow in the medium with inulin as a sole carbon source. The S. cerevisiae strain was constructed that is capable of heterologous expression of secreted K. marxianus inulase and is defective in fructose uptake due to null-mutations of the hexokinase structural genes HXK1 and HXK2. When grown in inulin-containing media, this strain is capable of accumulating at least 10% glucose-free fructose in the culture liquid. |
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