Functional analysis of a large non-conserved region of FlgK (HAP1) from Rhodobacter sphaeroides

The single subpolar flagellum of Rhodobacter sphaeroides shows an enlarged hook-filament junction. One of the two proteins that compose this section of the filament is HAP1 _Rs (FlgK _Rs ) it contains a central non-conserved region of 860 amino acids that makes this protein about three times larger than its homologue in Salmonella enterica serovar Typhimurium. We investigated the role of this central portion of the unusually large HAP1 protein of R. sphaeroides by monitoring the effects of serial deletions in flgK _Rs , the gene encoding HAP1 _Rs , on swimming and swarming. Two deletion mutants did not assemble functional flagella, two were paralyzed and five exhibited reduced free-swimming speeds. Some mutants produced unusual swarming patterns on soft agar without or with Ficoll 400. A segment of approximately 200-aa of the central region of HAP1 _Rs that aligns with the variable region of the flagellin sequence from other γ- and β-proteobacteria was also found. Therefore, it is possible that the origin of this large central domain of HAP1 _Rs could be associated with an event of horizontal transfer and subsequent duplications and/or insertions.