Thymic nuclear matrix associated activity is not V(D)J recombinase |
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| Authors: | V N Pandey V P Dave S B Amrute K Rosenbach M J Modak |
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| Affiliation: | Biochemistry Division, Bhabha Atomic Research Centre, Bombay, India. |
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| Abstract: | It was previously reported that nuclear matrix isolated from young rat thymus contained an activity that supported V(D)J recombination at a high efficiency (Dave et al., BIOCHEMISTRY 30: 4763-4767, 1991). A similar type of activity is also detected in the matrix prepared from fetal calf thymus. However, restriction enzyme mapping analyses of the recombined product clearly suggest that the double antibiotic resistance exhibited by the matrix treated plasmid substrate is not a consequence of V(D)J signal sequence recombination. |
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