Theobroxide Triggers Jasmonic Acid Production to Induce Potato Tuberization in vitro |
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Authors: | Xiquan Gao Fang Wang Qing Yang Hideyuki Matsuura Teruhiko Yoshihara |
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Affiliation: | (1) Division of Applied Bioscience, Graduate School of Agriculture, Hokkaido University, 060-8589 Sapporo, Japan;(2) Division of Bioresources and Crop Produce Science, Graduate School of Agriculture, Hokkaido University, 060-8589 Sapporo, Japan;(3) Present address: Department of Plant Pathology and Microbiology, Texas A&M University, College Station, 77843-2132, Texas, USA;(4) Present address: Department of Molecular Pharmacokinetics, Graduate School of Pharmaceutical Science, the University of Tokyo, 113-0033 Tokyo, Japan |
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Abstract: | Theobroxide, a novel compound isolated from fungus Lasiodiplodia theobromae culture, stimulates potato (Solanum tuberosum L.) tuber formation in vitro and in vivo, and induces flowering of Japanese morning glory (Pharbitis nil) under non-inductive long day conditions. To assess the mode of action of theobroxide in the tuberization process we measured endogenous levels of jasmonic acid and lipoxygenase activity in the cultures after the treatment with theobroxide. The results showed that theobroxide not only stimulated microtuber formation alone, but also enhanced the inductive effect of jasmonic acid when they were used in combination. The endogenous JA content increased in response to theobroxide in both old and new tissues. Moreover, theobroxide increased lipoxygenase activity in the cultures, at 2 and 3 week after culture initiation. Additionally, histological observations indicated that theobroxide might play a role in the swelling of micro-tubers formed in vitro in a similar manner as that of jasmonic acid. These results suggest that the inductive effect of theobroxide on potato micro-tuber formation might be achieved through triggering jasmonic acid production. |
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Keywords: | In vitro Jasmonic acid Lipoxygenase Potato tuberization Single-node segment culture Theobroxide |
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