Engineering of an N-acetylneuraminic acid synthetic pathway in Escherichia coli |
| |
| Authors: | Junhua Kang Pengfei Gu Yang Wang Yikui Li Fan Yang Qian Wang Qingsheng Qi |
| |
| Affiliation: | 1. State Key Laboratory of Microbial Technology, Shandong University, 27 Shanda Nanlu, Jinan 250100, PR China;2. National Glycoengineering Research Center, Shandong University, Jinan 250100, PR China;1. Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, Jiangnan University, Wuxi, 214122, China;2. Science Center for Future Foods, Jiangnan University, Wuxi, 214122, China;1. Department of Food Sciences, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor, Malaysia;2. Innovation Centre for Confectionery Technology (MANIS), Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor, Malaysia;3. Centre for Innovation and Technology Transfer (INOVASI@UKM), Chancellery, Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor, Malaysia;4. Department of Chemistry, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor, Malaysia;5. Department of Chemistry and Biomolecular Science, Faculty of Engineering, Gifu University, 1-1 Yanagido, Gifu 501-1193, Japan;1. Key Laboratory of Carbohydrate Chemistry & Biotechnology, Ministry of Education, Jiangnan University, Wuxi 214122, China;2. School of Biotechnology, Jiangnan University, Wuxi 214122, China;3. Department of Biotechnology & Food Technology, Faculty of Applied Sciences, Durban University of Technology, P.O. Box 1334, Durban 4001, South Africa;1. Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, Jiangnan University, Wuxi 214122, China;2. Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, Wuxi 214122, China;3. School of Chemical and Biomolecular Engineering, Georgia Institute of Technology, Atlanta 30332, USA;4. National Engineering of Laboratory for Cereal Fermentation Technology, Jiangnan University, Wuxi 214122, China |
| |
| Abstract: | N-acetylneuraminic acid (NeuAc) has recently drawn much attention owing to its wide applications in many aspects. Besides extraction from natural materials, production of NeuAc was recently focused on enzymatic synthesis and whole-cell biocatalysis. In this study, we designed an artificial NeuAc biosynthetic pathway through intermediate N-acetylglucosamine 6-phosphate in Escherichia coli. In this pathway, N-acetylglucosamine 2-epimerase (slr1975) and glucosamine-6-phosphate acetyltransferase (GNA1) were heterologously introduced into E. coli from Synechocystis sp. PCC6803 and Saccharomyces cerevisiae EBY100, respectively. By derepressing the feedback inhibition of glucosamine-6-phosphate synthase, increasing the accumulation of N-acetylglucosamine and pyruvate, and blocking the catabolism of NeuAc, we were able to produce 1.62 g l?1 NeuAc in recombinant E. coli directly from glucose. The NeuAc yield reached 7.85 g l?1 in fed-batch fermentation. This process offered an efficient fermentative method to produce NeuAc in microorganisms using glucose as carbon source and can be optimized for further improvement. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
