Screening of probiotic lactobacilli for inhibition of Shigella sonnei and the macromolecules involved in inhibition |
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| Authors: | Ying-Chun Zhang Lan-Wei Zhang Wei Ma Hua-Xi Yi Xin Yang Ming Du Yu-Juan Shan Xue Han Li-Li Zhang |
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| Institution: | 1. School of Food Science and Engineering, Harbin Institute of Technology, Harbin 150090, China;2. Heilongjiang Entry-Exist Inspection and Quarantine Bureau, Harbin 150086, China;1. Universidad Peruana Cayetano Heredia, Instituto de Medicina Tropical Alexander Von Humboldt, Lima, Peru;2. ISGlobal, Barcelona Ctr. Int. Health Res. (CRESIB), Hospital Clínic - Universitat de Barcelona, Barcelona, Spain;3. U.S Naval Medical Research Unit No.6, Callao, Peru;4. Center for Infectious Disease, University of Texas School of Public Health, Houston, USA;1. Institute of Disease Control and Prevention, Academy of Military Medical Sciences, 20 Dong-Da-Jie Street, Fengtai District, Beijing 100071, China;2. Institute of Basic Medical Sciences, 27 Tai-Ping-Lu Street, Haidian District, Beijing 100850, China;3. Clinical Diagnostic Center, 302 Hospital of PLA, 100 Xi-Si-Huan-Zhong-Lu Street, Fengtai District, Beijing 100039, China;1. Department of Global Health, University of Washington, Seattle, WA, USA;2. Department of Epidemiology, University of Washington, Seattle, WA, USA;3. Department of Pediatrics, University of Washington, Seattle, WA, USA;4. Department of Medicine, University of Washington, Seattle, WA, USA;5. Division of Infectious Disease, Department of Pediatrics, McMaster University, Hamilton, ON, Canada;1. Department of Food Technology, Laboratory of Food Microbiology, A.T.E.I. of Thessaloniki, Thessaloniki, P.O. Box 141, GR-57400, Greece;2. IASMA Research and Innovation Centre – Fondazione Edmund Mach, Food Quality and Nutrition Area Via E. Mach, 1, 38010 S. Michele (TN), Italy;3. Department of Food and Nutritional Sciences, University of Reading, Reading RG6 6AP, United Kingdom;1. Department of Animal Resources Science, Dankook University, Cheonan 31116, Republic of Korea;2. Department of Neurosurgery, Dankook University, Cheonan 31116, Republic of Korea |
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| Abstract: | A total of 91 lactobacilli were screened for antimicrobial activity against Shigella sonnei. Agar-well assay showed that 16 lactobacilli displayed strong antibacterial activity against S. sonnei. The nature of these antimicrobial agents were investigated and shown to be dependent on their production of organic acids. Adhesion tests showed that 6 lactobacilli demonstrated good adherence to HT-29 cells, of these Lactobacillus johnsonii F0421 were selected for acid and bile salt tolerance properties. We further research on L. johnsonii F0421 inhibition of S. sonnei adhesion to HT-29 cells. The result showed that L. johnsonii F0421 exhibited significant inhibitory activity and excluded, competed and displaced adhered S. sonnei by 48%, 38% and 33%, respectively. In order to elucidate the inhibitory functions of macromolecules involved in L. johnsonii F0421, the cells were treated with 5 m LiCl, 0.05 m sodium metaperiodate and heating and assayed for inhibition activity. The results suggested a role of S-layer proteins on L. johnsonii F0421 cells in inhibition of the adhesion process, but carbohydrates do not seem to be involved. SDS-PAGE analysis confirmed the presence of S-layer proteins with dominant bands of approximately 40 kDa. In addition, 100 μg/well of S-layer proteins from L. johnsonii F0421 cells were effective in inhibiting adhesion of S. sonnei to HT-29 cells. These findings suggest that L. johnsonii F0421 possesses the capacity for inhibition of S. sonnei activity as well as probiotic properties, which could serve as a potential novel and effective probiotic strain for use in the food industry. |
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