| SPF级KM小鼠与BALB/c小鼠肠道总菌多样性的比较 |
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| 引用本文： | 李伟,唐欢,周晓杨,魏泓. SPF级KM小鼠与BALB/c小鼠肠道总菌多样性的比较[J]. 中国微生态学杂志, 2010, 22(10): 865-867,875 |
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| 作者姓名： | 李伟 唐欢 周晓杨 魏泓 |
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| 作者单位： | [1]西南大学生命科学学院,重庆400715 [2]第三军医大学基础部实验动物学教研室,重庆400038 |
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| 基金项目： | 国家"973"计划 |
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| 摘 要： | 目的分析SPF级封闭群KM小鼠及近交系BALB/c小鼠的肠道菌群总菌多样性,比较两个不同遗传背景肠道总菌的丰富度、Shannon-Wiener指数和均匀度。方法收集SPF级KM小鼠和BALB/c小鼠新鲜粪便,提取粪便总菌DNA,用基于细菌16S rDNA序列的变性梯度凝胶电泳(PCR-DGGE)分析粪便总菌多样性。结果 SPF级KM小鼠及BALB/c小鼠粪便总菌多样性差异无统计学意义(P0.05),品系内不同性别之间粪便总菌多样性差异亦无统计学意义(P0.05)。结论选择SPF级小鼠进行微生态学相关研究时,封闭群KM小鼠及近交系BALB/c小鼠均可作为选择对象,同时可忽略菌群的性别差异。
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| 关 键 词： | KM小鼠 BALB／c小鼠 菌群多样性 PCR—DGGE |
The comparison of the microflora diversity between SPF degree KM mice and BALB/c mice |
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| LI Wei,TANG Huan,ZHOU Xiao-yang,WEI Hong. The comparison of the microflora diversity between SPF degree KM mice and BALB/c mice[J]. Chinese Journal of Microecology, 2010, 22(10): 865-867,875 |
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| Authors: | LI Wei TANG Huan ZHOU Xiao-yang WEI Hong |
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| Affiliation: | 1.College of Life Sciences,Southwest University,Chongqing 400715,China;2.Department of Animal Science,Collage of Basic Medicine,Third Military Medical University,Chongqing 400038,China) |
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| Abstract: | Objective To analyze the fecal microbiota of SPF KM mice and BALB/c mice,and compare the richness,Shannon-Wiener index and evenness of their total intestinal microflora.Method Fresh feces of SPF KM mice and BALB/c mice were collected.Total bacterial DNA of the fecal samples was extracted and 16S rDNA-based polymerase chain reaction-denaturing gradient gel electrophoresis（PCR-DGGE） was performed.Result No remarkable differences of diversity of total intestinal microflora were found between SPF KM mice and BALB/c mice（P〉0.05）,nor between different genders（P〉0.05）.Conclusion In the experiments related to the microbiota of SPF mice,KM mice and BALB/c mice were recommended equally and the difference of the gender could be neglected. |
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| Keywords: | KM mice； BALB/c mice； Microflora diversity； PCR-DGGE； |
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