Effects of culture media on hCTLA4Ig production and protein expression patterns in transgenic rice cell suspension cultures |
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Authors: | Dahyun Yu Mi-Na Song Jung-Ae Lim Dong-Il Kim |
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Institution: | (1) Department of Biological Engineering, Inha University, Incheon, 402-751, Korea |
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Abstract: | The effects of culture media on the production of human cytotoxic T-lymphocyte antigen 4-immunoglobulin (hCTLA4Ig) and intracellular
protein expression patterns were investigated in transgenic rice cell suspension cultures. Using comparative proteomic analysis,
changes in the intracellular proteome in different culture media were identified. Culture media were found to be an important
factor for the production of the recombinant target protein in this expression system, which was under the control of the
rice α-amylase 3D (RAmy3D) promoter. In terms of hCTLA4Ig production, the N6 medium produced a 3.7-fold higher level of protein
than the AA medium. In addition, the N6 medium provided better protein stability and cell viability. In the intracellular
proteome analysis, we identified eight proteomes that were differentially expressed. These results could provide valuable
information for the improvement of cell growth and target protein production. |
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Keywords: | rice cell suspension cultures hCTLA4Ig proteomics AA medium N6 medium |
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