Physical factors affecting the production of organic solvent-tolerant protease by Pseudomonas aeruginosa strain K |
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Authors: | Rahman Raja Noor Zaliha Abd Geok Lee Poh Basri Mahiran Salleh Abu Bakar |
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Institution: | Department of Biochemistry and Microbiology, Enzyme and Microbial Technology Research, Faculty of Science and Environmental Studies, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia. rnzaliha@fsas.upm.edu.my |
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Abstract: | The physical factors affecting the production of an organic solvent-tolerant protease from Pseudomonas aeruginosa strain K was investigated. Growth and protease production were detected from 37 to 45 degrees C with 37 degrees C being the optimum temperature for P. aeruginosa. Maximum enzyme activity was achieved at static conditions with 4.0% (v/v) inoculum. Shifting the culture from stationary to shaking condition decreased the protease production (6.0-10.0% v/v). Extracellular organic solvent-tolerant protease was detected over a broad pH range from 6.0 to 9.0. However, the highest yield of protease was observed at pH 7.0. Neutral media increased the protease production compared to acidic or alkaline media. |
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