Genes up-regulated during red coloration in UV-B irradiated lettuce leaves |
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Authors: | Jong-Sug Park Myoung-Gun Choung Jung-Bong Kim Bum-Soo Hahn Jong-Bum Kim Shin-Chul Bae Kyung-Hee Roh Yong-Hwan Kim Choong-Ill Cheon Mi-Kyung Sung Kang-Jin Cho |
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Institution: | (1) Research Planning & Information Division, National Institute of Agricultural Biotechnology, Suwon, 441-707, Korea;(2) Molecular Physiology and Biochemistry Division, National Institute of Agricultural Biotechnology, Suwon, 441-707, Korea;(3) Department of Pharmacognosy Material Development, Kangwon National University, Samcheok, 245-711, Korea;(4) Department of Life Science, Sookmyung Women’s University, Youngsan-gu, Seoul, 140-742, Korea;(5) Department of Food and Nutrition, Sookmyung Women’s University, Youngsan-gu, Seoul, 140-742, Korea |
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Abstract: | Molecular analysis of gene expression differences between green and red lettuce leaves was performed using the SSH method.
BlastX comparisons of subtractive expressed sequence tags (ESTs) indicated that 7.6% of clones encoded enzymes involved in
secondary metabolism. Such clones had a particularly high abundance of flavonoid-metabolism proteins (6.5%). Following SSH,
566 clones were rescreened for differential gene expression using dot-blot hybridization. Of these, 53 were found to overexpressed
during red coloration. The up-regulated expression of six genes was confirmed by Northern blot analyses. The expression of
chalcone synthase (CHS), flavanone 3-hydroxylase (F3H), and dihydroflavonol 4-reductase (DFR) genes showed a positive correlation with anthocyanin accumulation in UV-B-irradiated lettuce leaves; flavonoid 3′,5′-hydroxylase (F3′,5′H) and anthocyanidin synthase (ANS) were expressed continuously in both samples. These results indicated that the genes CHS, F3H, and DFR coincided with increases
in anthocyanin accumulation during the red coloration of lettuce leaves. This study show a relationship between red coloration
and the expression of up-regulated genes in lettuce. The subtractive cDNA library and EST database described in this study
represent a valuable resource for further research for secondary metabolism in the vegetable crops. |
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Keywords: | Lettuce Anthocyanin EST Flavonoid Red coloration SSH UV-B Northern blot |
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