Histochemical demonstration of enzymes related to NADPH-dependent hydroxylating systems in rat liver after phenobarbital treatment |
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Authors: | J Koudstaal M J Hardonk |
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Institution: | (1) Dept. of Pathology, University of Groningen, The Netherlands |
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Abstract: | Summary Male rats were given 100mg phenobarbital for three days intraperitoneally. Biochemically an increase was found in activity of nitro-anisole demethylation and in the content of cytochrome P-450. Enzymhistochemically an increase in activity was noted for NADPH tetr. red., G6PD, ICD, and Naftol AS-D-esterase; a decrease was seen in G6Pase and glycogen, but no difference was found in NADH tetr. red. From these results it has been suggested that NADPH tetr. red. is directly involved in the hydroxylation chain, while G6PD and ICD are more indirectly involved.List of Abbreviations NADH
nicotinamide adenine dinucleotide
- NADPH
nicotinamide adenine dinucleotide phosphate
- NADPH tetr. red.
NADPH tetrazolium reductase
- G6PD
glucose-6-phosphate dehydrogenase
- ICD
iso-citric acid dehydrogenase
- G6Pase
glucose-6-phosphatase
- PAS
periodic acid-Schiff method |
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