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Growth requirements and the establishment of a chemically defined minimal medium inZymomonas mobilis
Authors:Irene Galani  Constantin Drainas  Nilton A. Typas
Affiliation:(1) Department of Biochemistry, Athens University, Panepistemiopolis, T.K. 15701 Athens, Greece;(2) Department of Mollecular and Cellular Biology, Athens University, Panepistemiopolis, T.K. 15701 Athens, Greece;(3) Department of Genetics, Athens University, Panepistemiopolis, T.K. 15701 Athens, Greece;(4) Department of Chemistry, Division of Organic Chemistry and Biochemistry, University of Ioannina, Ioannina, Greece
Abstract:Summary A chemically defined minimal medium which fulfils the growth requirements of differentZymomonasmobilis strains has been established. The kinetics of ethanol production of the strains ATCC 10988, CU1, CP4 and 11163 grown on the minimal medium at different glucose concentrations were measured. All strains produced ethanol at rates similar to those on complete medium. The minimal medium described is suitable to study spontaneous metabolic deficiciencies and regulation of enzyme activities inZ.mobilis.
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