Monitoring expression profiles of Arabidopsis genes during cold acclimation and deacclimation using DNA microarrays |
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| Authors: | Youko Oono Motoaki Seki Masakazu Satou Kei Iida Kenji Akiyama Tetsuya Sakurai Miki Fujita Kazuko Yamaguchi-Shinozaki Kazuo Shinozaki |
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| Affiliation: | (1) Plant Functional Genomics Research Group, RIKEN Genomic Sciences Center, 1-7-22 Suehiro-cho, Tsurumi-ku Yokohama, 230-0045, Japan;(2) Graduate School of Life and Environmental Science, University of Tsukuba, 1-1-1 Tennoudai, Tsukuba Ibaraki, 305-0074, Japan;(3) Laboratory of Plant Molecular Biology, RIKEN Tsukuba Institute, 3-1-1 Koyadai, Tsukuba 305-0074, Japan;(4) Biological Resources Division, Japan International Research Center for Agricultural Sciences (JIRCAS), 2-1 Ohwashi, Tsukuba Ibaraki, 305-8686, Japan;(5) CREST, Japan Science and Technology Corporation (JST), Tokyo, Japan;(6) Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyou-ku, Tokyo 113-8657, Japan;(7) RIKEN Plant Science Center, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama 230-0045, Japan |
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| Abstract: | A comparative analysis of gene expression profiles during cold acclimation and deacclimation is necessary to elucidate the molecular mechanisms of cold stress responses in higher plants. We analyzed gene expression profiles in the process of cold acclimation and deacclimation (recovery from cold stress) using two microarray systems, the 7K RAFL cDNA microarray and the Agilent 22K oligonucleotide array. By both microarray analyses, we identified 292 genes up-regulated and 320 genes down-regulated during deacclimation, and 445 cold up-regulated genes and 341 cold down-regulated genes during cold acclimation. Many genes up-regulated during deacclimation were found to be down-regulated during cold acclimation, and vice versa. The genes up-regulated during deacclimation were classified into (1) regulatory proteins involved in further regulation of signal transduction and gene expression and (2) functional proteins involved in the recovery process from cold-stress-induced damages and plant growth. We also applied expression profiling studies to identify the key genes involved in the biosynthesis of carbohydrates and amino acids that are known to play important roles in cold acclimation. We compared genes that are regulated during deacclimation with those regulated during rehydration after dehydration to discuss the similarity and difference of each recovery process.Electronic Supplementary Material Supplementary materials are available for this article at |
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| Keywords: | Deacclimation Cold acclimation Recovery from cold stress Cold stress |
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