Binding of colchicine to a soluble fraction of carrot cells grown in suspension culture |
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Authors: | Shoji Okamura |
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Affiliation: | (1) Faculty of Pharmaceutical Sciences, Toyama Medical and Pharmaceutical University, Sugitani, 930-01 Toyama, Japan |
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Abstract: | The binding of [3H]colchicine to soluble component prepared from carrot (Daucus carota L.) cells in suspension culture was assayed by the diethylaminoethyl(DEAE)-cellulose powder method. The binding activity was very labile and the time course of the binding indicated that the colchicine-bound complex was also unstable. The reaction was enhanced by vinblastine but lumicolchicine had no effect. The optimum temperature for the reaction was 30° C, and the colchicine binding constant was calculated to be 3.5·104 l mol–1 at 30° C. |
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Keywords: | Cell culture Colchicine Daucus Tubulin |
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