Secretion of granule enzymes from alveolar macrophages : Regulation by intracellular Ca-buffering capacity |
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Authors: | C. Schneider R. Gennaro G. de Nicola D. Romeo |
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Affiliation: | Department of Biochemistry, University of Trieste, 34127 Trieste, Italy |
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Abstract: | Rabbit alveolar macrophages exposed to the ionophore A23187 in the presence of extracellular Ca2+ take up about 12 nmoles of Ca2+/1 × 106 cells. This uptake induces a slight, but significant, extracellular release of granule enzymes, β-glucuronidase and lysozyme, but not of the cytoplasmic marker, lactate dehydrogenase. If either EGTA is added to the medium or Mg2+ replaces Ca2+ no stimulation of secretion is observed. If the energy supply is decreased by treating the macrophages with mitochondrial inhibitors such as oligomycin, cyanide, or rotenone and antimycin, Ca2+-dependent secretion is potentiated several fold. Selective release of granule enzymes from macrophages exposed to A23187 and Ca2+ is also stimulated by cytochalasin B (CB). |
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