酶固定化的新型载体──PF凝胶应用研究

用对苯二酚和甲醛在酸催化下制得新一类凝胶，此凝胶价廉，易于制备，多孔、无毒、亲水性强；能选择性吸附多糖，而用于多糖与低聚糖及单糖的分离；可作为载体对多种酶及蛋白质给予固定，与蛋白质的最大结合量为558mg/g,固定糖化酶时的活力回收达84%;此固化酶对淀粉的转化率达93%,当加入一定量的间苯二酚,可得改性的PF凝胶,其性能又有提高,可见PF凝胶为新型的酶的固定化优良载体.

Application of PF Gel as New Carrier for the Immobilization of Enzyme

PF gel-type resin has been synthesised by poly-condensation of hydroquinone with formaldehyde in the presence of acidic solution.The gel-type resin is cheap and easy to preduce,non-toxic,porous,hydrophilic, extremely stable.It is a effective carrier for the simple and rapid immobilization of various enzymes and proteins.The amounts of bound protein (BSA) and glycoamylase in one gramme of the dry carrier were 558mg and 330mg respectively,the activity recovery of 84% for immobilized glycoamylase was obtained. The coversion(%)for starch into glycoase, using immobilized glycoamylase is as high as 93%.A new kind of modified PF gel-type resins were synthesised by copolymerization of hydroquinone and some resorcin with formaldehyde. Thus PF and modified PF gel-type resins are superior carriers for immobilization of enzyme.