| 目前最高分辨率的电泳──固相pH梯度等电聚焦 |
| |
| 引用本文： | 郭尧君,郭强.目前最高分辨率的电泳──固相pH梯度等电聚焦[J].生物化学与生物物理进展,1994,21(2):143-146. |
| |
| 作者姓名： | 郭尧君 郭强 |
| |
| 作者单位： | 中国科学院生物物理研究所;中国科学院生物物理研究所 |
| |
| 摘 要： | 固相pH梯度等电聚焦是国际上80年代的新型电泳技术.利用一系列具有弱酸和弱碱性质的丙烯酰胺衍生物滴定时，在滴定终点附近形成的pH梯度并参与丙烯酰胺的共价聚合，从而形成固定的不随环境电场等条件变化的pH梯度，该方法具有比传统载体两性电解质等电聚焦更高的分辨率、更大的上样量.可用于分析和制备相近pI的蛋白质，多肽等.
|
| 关 键 词： | 等电聚焦 固相pH梯度 载体两性电解质 |
| 收稿时间： | 1993/9/24 0:00:00 |
| 修稿时间： | 1993/11/17 0:00:00 |
A Current Break Through Electropboresis Technique with Superinost Resolution: Immobilized pH Gradients Isoeltctrofocusing |
| |
| Guo Yaojun and Guo Qiang.A Current Break Through Electropboresis Technique with Superinost Resolution: Immobilized pH Gradients Isoeltctrofocusing[J].Progress In Biochemistry and Biophysics,1994,21(2):143-146. |
| |
| Authors: | Guo Yaojun and Guo Qiang |
| |
| Institution: | Institute of Biophysics. CAS;Institute of Biophysics. CAS |
| |
| Abstract: | The mentioned immobilized PH gradients isoelectrofocusing is a electrophoresis technique developed in 80' s. An approximate linear pH gradient is genarated by titrating weak acidic and basic acrviamide derivaties which then covalent bound into the polyacrylamide matrix. The pH gradient is stable and independent of electric field fluctuation. The present method provides higher resolution and larger loading capacity comparing with conventional carrier ampholyte isoeletrofocusing. It can analyse and purify protein with only minor pI difference. |
| |
| Keywords: | isoelectrofocusing (IEF) immo-bilized PH gradient(IPG) carrier amphelyte(CA) |
| 本文献已被 CNKI 维普 等数据库收录！ |
| 点击此处可从《生物化学与生物物理进展》浏览原始摘要信息 |
| 点击此处可从《生物化学与生物物理进展》下载免费的PDF全文 |
