首页 | 本学科首页   官方微博 | 高级检索  
   检索      


Elicitation of different Panax ginseng transformed root phenotypes for an improved ginsenoside production
Authors:Javier Palazn  Rosa M Cusid  Mercedes Bonfill  Anna Mallol  Elisabet Moyano  Carmen Morales  M Teresa Piol
Institution:a Laboratorio de Fisiología Vegetal, Facultad de Farmacia, Universidad de Barcelona, Avda. Diagonal 643, 08028, Barcelona, Spain;b Departament de Ciències Experimentals i de Salut, Universitat Pompeu Fabra, Avd. Dr. Aiguader 80, 08003, Barcelona, Spain
Abstract:We tested the effect of the presence in the culture medium of chitosan, vanadyl sulfate or methyl jasmonate on growth and ginsenoside production of three stable hairy root lines of Panax ginseng C.A. Meyer showing different morphological phenotypes C-M, HR-M and T-M. The response depended upon line phenotype, specificity of the elicitor and the stage of growth at which the lines were treated. The highest ginsenoside yield was found when methyl jasmonate was added during the progressive deceleration growth phase (on day 25 of culture). In this case, the ginsenoside content reached at the end of the culture (day 28) by root lines C-M, HR-M and T-M was, respectively, 2, 1.8 and 4 times higher than the highest content achieved, also at the end of the culture, by the corresponding untreated root lines. Under the same conditions, the ginsenoside content in the presence of vanadyl sulfate also increased considerably, while with chitosan it clearly decreased. The ginsenoside pattern in response to the presence of the elicitors is also considered.
Keywords:Chitosan  Elicitation  Ginsenosides  Hairy root cultures  Methyl jasmonate  Panax ginseng  Vanadyl sulfate
本文献已被 ScienceDirect 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号